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KMID : 0613820130230091140
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Journal of Life Science
2013 Volume.23 No. 9 p.1140 ~ p.1146
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Protective Effect of Dried Mackerel Extract on Lipopolysaccharide-induced Inflammation
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Kim Kwang-Hyuk
Choi Myoung-Won
Choi Hyang-Mi
Lim Sun-Young
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Abstract
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The effect of dried mackerel extract on the production of nitric oxide (NO) and cytokines, including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interferon-r (IFN-r), was investigated. All extracts and fractions from dried mackerel significantly reduced NO production induced by lipopolysaccharide (LPS). Among the extracts, acetone+methylene chloride (A+M), n-hexane, and 85% aqueous methanol (MeOH) showed the strongest inhibitory effects. The 85% aqueous MeOH fraction at a concentration of 10μg significantly decreased LPS-induced IL-6 and TNF-α production after 6 hr of incubation. In the case of LPS-induced IFN-r production, the 85% aqueous MeOH fraction decreased the production of IFN-r afer 6, 24, and 72 hr of incubation in a dose-dependent manner. The results show that an 85% aqueous MeOH fraction inhibits the production of NO and proinflammatory cytokines (IL-6, TNF-α, IFN-r), suggesting that this fraction acts as a potent immunomodulator.
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KEYWORD
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Interferon-r, interleukin-6, mackerel extract, nitric oxide, tumor necrosis factor-α
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